Monday, January 12, 2015

Objective(s):
Connect the laboratory techniques of Biology to identification of prokaryotic cells
Learn techniques used in a Biology Lab (Pipetting, PCR, Gel Electrophoresis)

Agenda:
The Case of the Tainted Pattie
What is DNA?
How can we use it to identify organisms? (Brief overview of PCR, Restriction Enzymes and Gel Electrophoresis)
Interactive: DNA Extraction
Video: Chromosome 11 Flyover


Homework:
Final Proposal DUE FRIDAY!!!
Review Sheet #2, part 2
Research Project Data Collection DATA CHECK ONE ON TUESDAY NEXT WEEK!!!


Tuesday, January 13, 2015

Objective(s):
Explore the Techniques and tools of DNA

Agenda:
Micropipette Introduction
Micropipette Resource Video 1
Micropipette Resource Video 2
Micropipette Resource Video 3
Students complete tasks using micropipettes

Homework:
Continue to work on:
1) Data Collection for Research Project
2) Research Project Proposal Final Version
3) Study Guide for Mid Year Exam


Wednesday, January 14, 2015 (Early Release)
Objective(s):
Understand the steps and reagents used in PCR, Restriction Digest and Gel Electrophoresis

Agenda:
1) Review components for PCR, Restriction Digest, and Gel Electrophoresis
2) Review Protocol Instructions and Materials


Homework:
Study Guide for Mid Year Exam - Chapter 7, Part 3


Thursday, January 15, 2015
Objective(s):
Perform PCR and Restriction Digest of a sample of DNA

Agenda:
Gather Materials
Review Safety and Communicate Hazards
Perform PCR
Video 1
Video 2
Question to ponder - How do cellular replication and PCR (amplification) compare and contrast?

Homework:
Collect DATA for Research Project

Friday, January 16, 2015
Objective(s):
Perform a Gel Electrophoresis of the Sample
Complete Analysis of your experiment

Agenda:
Review Safety and Communicate Hazards
Retrieve samples and reagents for today's activity
Perform Restriction Enzyme Digest
Store samples for Tuesday's Gel Electrophoresis

Homework:
Collect DATA for Research Project